• Protein Biochemistry. We provide carefully designed, high-quality and thoroughly characterized proteins for screening and assay development. Leveraging our expertise across target classes (including integral membrane proteins & multi-subunit complexes) and utilizing both internal & external resources we partner directly with teams to produce proteins that are qualified for even the most challenging applications.
• Biophysics & Biochemistry. Using a toolbox of techniques including SPR, fluorescence/CD spectroscopy, calorimetry, nano-DSF & radioligand binding we perform detailed thermodynamic & kinetic profiling of protein-ligand interactions across all modalities.
• BioNMR. Our capabilities in high-resolution NMR spectroscopy enable us to rigorously study the solution behavior of proteins (including excipient and self-association effects) and to conduct detailed experiments probing molecular interactions using both ligand- and protein-detected techniques.
• Mass Spectrometry. We specialize in biophysical mass spectrometry including HDX-MS for protein characterization, binding site mapping and epitope/paratope determination; metabolic flux as a readout for pharmacodynamics and pathway engagement in physiologically relevant settings (both in vitro and in vivo); and ultrahigh-throughput mass spectrometry where we have partnered with groups across the network to enable screening, chemical reaction profiling and protein engineering.
• Our overarching goal is to work directly with teams to accelerate the discovery and characterization of molecules on the path to delivering meaningful therapies to patients.

Core responsibilities:
• Run routine radioligand binding assay protocols, analyze data & generate weekly reports, carry out tissue culture and cell-based binding assays.
• Fully document all experiments in our electronic laboratory notebook.

Must Haves/Required skills:
• Prior experience developing equilibrium and kinetic radioligand binding assays (e.g. 125I, 3H, 35S) in relevant formats is preferred, or willingness to do radioligand work.
• Tissue culture including: (i) cell plating, (ii) cell stimulation and (iii) cell harvesting.
• Experience in membrane preparation
• Experience working with multiwell plates (96- and 384-well formats)
• Note that this position is laboratory-based.

Nice to haves/Preferred skills:
• Experience with liquid handling automation systems (e.g. Echo, Bravo).
• Familiarity with multi-mode plate readers such as the SpectraMax and EnVision Multilabel Reader.
• Exporting and dose-response fitting of data from binding assays.
• Running research operating plan assay and data upload (ROP assay, ActivityBase application)
• Experience with traditional Western blot techniques, including requisite data analysis used to determine abundance of given protein targets. Knowledge of the Protein Simple high-throughput, automated western blotting system is preferred but not required.


EEO/AA Employer/Vet/Disabled.

The Know Your Rights poster is available here:
https://www.eeoc.gov/sites/default/files/2023-06/22-088_EEOC_KnowYourRights6.12.pdf

The pay transparency policy is available here:
https://www.dol.gov/sites/dolgov/files/ofccp/pdf/pay-transp_%20English_formattedESQA508c.pdf

For temporary assignments lasting 13 weeks or longer, the Company is pleased to offer major medical, dental, vision, 401k and any statutory sick pay where required.

We are committed to working with and providing reasonable accommodations to individuals with disabilities. If you need a reasonable accommodation for any part of the employment process, please contact your staffing representative who will reach out to our HR team.

AllSTEM Connections participates in the E-Verify program in certain locations as required by law. Learn more about the E-Verify program.
https://e-verify.uscis.gov/web/media/resourcesContents/E-Verify_Participation_Poster_ES.pdf

We also consider qualified applicants regardless of criminal histories, consistent with legal requirements. Pursuant to applicable state and municipal Fair Chance Laws and Ordinances, we will consider for employment-qualified applicants with arrest and conviction records. For Los Angeles, CA applicants: Qualified applications with arrest or conviction records will be considered for employment in accordance with the Los Angeles County Fair Chance Ordinance for Employers and the California Fair Chance Act.